| Solution Information | help | |
| Enzyme: | Serine/threonine-protein phosphatase | |
| inhibitor: | BDBM60225 | |
| substrate: | n/a | |
| Solution Type: | Aqueous | |
| pH at Preparation: | n/a | |
| Temp. Prep.: | n/a | |
| Comments: | 1) Grow reporter strain in SD-URE media overnight @ 23 degrees C. 2) Dilute cells to OD = 0.01 in SD-URE media. 3) Add 35 ul of culture to each well of 384 white plate. 4) Pin 100 nL compounds into plates, using 5uM FK506 as positive control. 5) Incubate plates for 75 minutes at 23C, with agitation. 6) Add 5 ul of SD-URA with 1.25M Calcium chloride to plates. 7) Incubate at 30 C for 90 minutes, with agitation. 8) Add 40 ul Gal-Screen reagent. 9) Incubate at 30C for 25 minutes, then read luminescence. | |
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